Recombinant Lysyl Endopeptidase

   Lysyl Endopeptidase (EC 3.4.21.50) belongs to a serine protease that specifically cleaves peptide bonds at the carboxy terminus of lysine residues. Lysyl Endopeptidase is widely used in biopharmaceutical, biological analysis, cell culture and other fields.

   This product is a lyophilized powder expressde by recombinant E. coli, freeze-dried by multi-step separation and purification, has the same activity and specificity as natural lysyl endo-enzyme. This product is a biochemical grade genetically engineered enzyme which does not contain enzyme inhibitors such as DFP, PMSF and TLCK. Serine protease inhibitors such as PMSF can inhibit enzyme activity, but metal ion chelators such as EDTA do not  inhibit enzyme activity. This product is an unlabeled Lysyl Endopeptidase with the same amino acid sequence as the native Lysyl Endopeptidase. 

★ Advantages

l   No animal origin：Free from virus contamination and does not use any animal derived materials during the production process.

l   Meet BP2019 and EP8.8 standards.

l   Strong tolerance：wide range of pH tolerance and strong surfactant tolerance.

l   Consistent quality：It can guarantee continuous and stable mass production, and the quality difference between batches is small.

l   Compliance：Production equipment and production environment must comply with relevant regulations and comply with GMP guidelines.

l   Complete quality documentation：Relevant regulatory support documents are available upon request.

★ Properties

Enzymatic Unite: 30 ° C, pH 9.5, the enzyme amount of 1 μmol of p-nitroaniline per minute of catalytic substrate was 1 AU.

★ Application

l   Used for the production of biological products, such as insulin and analog production, GLP-1 analogue production.

l   Used for protein structure and function studies, such as protein mass spectrometry, sequencing, peptide mapping analysis.

l   Used for production of small molecular proteins or peptides, such as cosmetic peptides, antimicrobial peptides, food flavor peptide production.

l   Used for enzymatic synthesis for Lys-X.

l   Used for cultivation of stem cells and primary cells, with milder effecttion.

★  Usage method

l      It is recommended to dissolve the enzyme using 20mM Tris of pH8-10. Dissolve the protein or peptide in buffer,and adjust the pH to the optimum pH 9.0-10.0，then add Lys-C with ratio of 2~100AU/g peptide or protein and incubate sample for 2~18 hours at 25~ 35°C. 

l      Precautions：Inorganic salts above 0.1M have a certain effect on the enzyme activity, and it is recommended to carry out the enzyme digestion reaction after desalting. If it is not possible to desalinate, it is recommended to increase the amount of enzyme and extend the digestion time.

★  Stability

l   Transport stability：Insulation with ice packs to ensure stable enzyme activity. 

l    Stored at -15 ° C to -20 °C and can be stable for 24 months. When Tris-HCl buffer is formulated    into an enzyme solution,repeated freezing and thawing at -20℃ for 10 times without activity loss, and  stable storage for a week at 4℃ and -20℃.

★ Related products  

      Recombinant Lysyl Endopeptidase  Lyophilized powder(with His Tag)lyophilized powder；Recombinant Trypsin Lyophilized powder；Recombinant Carboxypeptidase B (with His Tag) lyophilized powder. 

★ Specifications