Recombinant Human Epidermal Growth Factor (EGF)

Recombinant human epidermal growth factor (EGF) is a biologically active protein factor in cell culture, type of polypeptide substance widely present in mammals, which exert a physiological action by binding to the EGF receptor on the cell membrane. EGF has a profound effect on the differentiation of specific cells in vivo, and is a powerful mitotic factor in a variety of in vitro cultured ectoderm and mesoderm cells.In vitro, EGF is a mitogen of fibroblasts, epithelial cells, and endothelial cells, promoting colony formation in epithelial cell culture.In vivo, EGF promotes the formation of epithelial cells and blood vessels. 

This product is a lyophilized powder prepared by recombinant Pichia pastors, separated and purified by multiple steps, and then sterilized by filtration through a 0.22 μm filters and freeze-dried.

- Product Application

        It is widely used in cell culture of fibroblasts, keratinocytes, epithelial cells and endothelial cells.

- Properties

- Recommended Method

       It is recommended to centrifuge the powder to the bottom of the tube before opening the lid, then dissolve it in sterilized deionized water to prepare a storage solution of 100 μg/ml. It can be stored stably for 2 weeks at 2~ 8 ℃. When diluted to a lower concentration (not less than 10μg/ml), 0.1% tissue culture grade BSA or HSA is required. If it is necessary to extend the storage time, it is recommended to freeze at -80 ℃ or -20 ℃ rather than multigelation. This product should be determined by experiment to determine the optimum concentration according to each specific application.

- Transport and Storage Stability

       It is recommended to centrifuge the powder to the bottom of the tube before opening the lid, then dissolve it in sterilized deionized water to prepare a storage solution of 100μg/ml. It can be stored stably for 2 weeks at 2°C~8°C. When diluted to a lower concentration (not less than 10 μg/ml), 0.1% BSA or HSA is required. If1. Transportation stability: It is transported in an ice pack to keep it active. 

      Storage stability: stable to 12 months at -15~-20°C, stable to 4 weeks at 2~8°C.Prepared as a storage solution of 100μg/ml, stable storage at 2~8°C for one week.

- Notice and Disclaimer

      1. Avoid long time storage in high temperature environment 

      2. Avoid contamination during operation of this product. 

      3. The product is for research use ONLY. DO NOT use it for diagnosis or treatment of animals or humans.