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Recombinant human insulin-like growth factor-1（IGF-1）

CAS：62031-54-3
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- Description

Recombinant human basic fibroblast growth factor (also known as basic FGF, bFGF, FGF2, FGF-β or heparin-binding growth factor) is a biologically active protein suitable for cell culture applications. The bFGF regulates various processes such as cell proliferation, differentiation, survival, adhesion, exercise, apoptosis, limb formation and wound healing. The bFGF can be used for angiogenesis, fibroblast mitosis, axonal growth of PC-12 cells, receptor binding and tyrosine phosphorylation studies.

The product is lyophilized powder prepared by recombinant E.coli expression, separated and purified by multiple steps, dissolve in 10mM PBS, 0.15 M NaCl (pH7.2) solution and then sterilized by filtration through a 0.22μm filter membranes and freeze-dried.

- Properties

Appearances	White or off-white lyophilized powder
Species	Human
Source	E.coli
MW	17.3KD±1.7kDa
Purity	≥95%（SDS-PAGE）
Endotoxin	< 0.2 EU/µg
Aseptic	Filter and sterilize with 0.22μm filter before freeze-drying
Isoelectric point	9.5-9.6
Activity	≥5.0 x 106IU /mg pro.
Solubility	Good solubility in water, buffer, and medium

Biological activity: The biological activity of the mouse fibroblast cell line BALB/3T3 was

determined by the method of Chinese Pharmacopoeia 2020 edition using bFGF.

-Product Application

Used in cell culture to enhance cell proliferation, cell growth and cell differentiation.

- Transport and Storage Stability

Transportation stability: It is transported in an ice pack to keep it active.

Storage stability: stable to 12 months at -15~-20°C and 24 months at -80°C, stable to 4 weeks at 2~8℃. Prepare as a storage solution of 100μg/ml, stable storage at 2~8℃ for one week.

- Recommended Method

It is recommended centrifuging the powder to the bottom of the tube before opening the lid, then dissolve it in sterilized demonized water to prepare a storage solution of 100 μg/ml. It can be stored stably for 2 weeks at 2℃ ~ 8℃.When diluted to a lower concentration (not less than 10μg/ml), 0.1% tissue culture grade BSA or HSA is required. If it is necessary to extend the storage time, it is recommended to freezing at -80℃ or -20℃ rather than multigelation.This product should be established by experiment to determine the optimum concentration according to each specific application.

- Notice and Disclaimer

All product should be stored away from moisture, direct sunlight, and heat.

Avoid contamination during operation of this product.

All product is for research use ONLY. DO NOT use it for diagnosis or treatment of animals or humans.

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